Brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation

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Brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation.

The purpose of this study was to determine whether defined dietary manipulations would enhance colonization of mice experimentally challenged with the intestinal spirochaete Brachyspira pilosicoli. Weanling C3H/HeJ mice (n = 48) were fed either a standard balanced mouse diet or a diet supplemented with 50 p.p.m. zinc bacitracin (ZnB), with 50 % (w/w) lactose or with both supplements. Eight mice...

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Evaluation of PCR amplification from fixed caecal tissue to determine the infection of experimentally infected laying hens with Brachyspira pilosicoli

Avian intestinal spirochaetosis (AIS) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus Brchyspira. The purpose of the present study was to evaluate PCR amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with Brachyspira pilosicoli  with the same primers that have been used for det...

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Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilit...

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Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea estates in Assam, India.

The prevalence of colonization with the anaerobic intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli was investigated in humans (n = 316) and dogs (n = 101) living on three tea estates in Assam, India. Colonization was detected using PCR on DNA from faeces. Nineteen (6%) human faecal samples contained B. aalborgi DNA, 80 (25.3%) contained B. pilosicoli DNA, and 10 (3.2%) co...

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Vaccination with an autogenous bacterin fails to prevent colonization by Brachyspira intermedia in experimentally infected laying chickens.

Avian intestinal spirochaetosis (AIS) is a disease complex affecting adult laying and breeding chickens associated with infection by anaerobic intestinal spirochaetes of the genus Brachyspira. Options for control of AIS are limited, as few effective antimicrobial agents are registered for use in laying chickens. One of the two most commonly encountered pathogenic species in AIS is B. intermedia...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 2004

ISSN: 0022-2615,1473-5644

DOI: 10.1099/jmm.0.05393-0